Diffusion rate of methylene blue on agar plates at different temperatures essay

To test the effect of molecular weight on the rate of diffusion, various experiments were performed one of which is the glass tube test wherein cotton balls of the same size were moistened in two different substances (nh4oh and hcl. Lab 1 safety rules and bacteria in the environment 8/28/02 3 agar plates of levine eosin-methylene blue (emb) for example, heating a bacterial culture at different temperatures gives: the following example illustrates the implications of exponential killing suppose you are canning chicken soup. Diffusion of dyes (rhodamine 6g and methylene blue) in gels of agar was investigated at three temperatures (30, 50, 70 °c) until equilibrium was reached ways to analyse and model the experimental data were presented and the diffusion coefficients of the dyes were estimated using fick’s second law of diffusion. The ability of the isolate to grow on different mediums was tested on eosin methylene blue, macconkey agar, and blood agar plates using given protocol (lab 8 handout) genotypic tests: dna was extracted using powersoil dna isolation kit (mo bio laboratories, a qiagen co.

diffusion rate of methylene blue on agar plates at different temperatures essay At the end of the 5-h incubation period, the viable count had declined from a starting value of 16×10 6 cfu ml −1 to undetectable on the selective agar plates mlsa and to a value of 63 cfu ml −1 on the non-selective agar plates na.

In lab, we were seeing the diffusion of agar we had 3 plates agar a, b, and c we put a drop of potassium permangante and a drop of methylene blue and measured at a specific time the diameter of each solution. Discussion 1 given the molecular weights of methylene blue, ferric chloride, and potassium permanganate, what can we conclude from the relationship of molecular weight to the rate of diffusion. Characterized for transition temperatures, rheological, mechanical, and mucoadhesive methylene blue ndds novel drug delivery system nsaids non-steroidal anti-inflammatory drugs diffusion rate of drug hydrogels hydrogel is a transparent, viscoelastic and thermo dynamically stable system consisting. The plates were then allowed to solidify after which, four agar wells were made in each of the plates seeded with the indicator bacteria the seeded plates were prepared in sets that corresponded to the purified lab isolates whose bacteriocins were being investigated for antimicrobial activity.

A collect agar cubes of different sizes – 2 cm x 2 cm, 1 cm x 1 cm and 05 cm x 05 cm – or cut cubes from the larger cube provided b place the cubes in a beaker and cover with the diffusing solution. Are resistant to high temperatures, uv light, and desiccation question 125 assume you are growing a bacterial culture in a glucose medium your lab partner adds more glucose in hopes of speeding up the experiment. Three blue colonies on mi agar were not e coli, giving a false-positive rate of 43% (3 of 69 colonies), and three organisms that demonstrated fluorescence but were not blue (ie, tc) were actually e coli, resulting in a false-negative rate of 43% (3 of 69 colonies. The ditch plate method of agar diffusion used by alexander fleming was the clarity and/or color are enhanced by heat and diffusion of light elements such as will be provided to each student, therefore, the emphasis of this lab it not [89. Timing the disappearance of the blue colour at different temperatures 201 effect of temperature 202 discussion 203 discussion - answers 2 diffusion in liquid diffusion of methylene blue through gelatine is observed 201 diffusion in liquid the potometer is used in different conditions to compare rates of uptake by the shoot 301.

The diffusion rate of the blue food color at two different temperatures 1) obtain 2 agar plates use a cut 3ml pipette to make a well in the center of both plates. Abstractthe effect of the temperature of the medium on the rate of diffusion of potassium permanganate (kmno4), was determined with. The shelf life of mueller-hinton agar supplemented with 2% glucose and methylene blue (05 μg/ml) (mh-gmb) prepared in the laboratory to test disk diffusion of voriconazole and fluconazole was.

Diffusion rate of methylene blue on agar plates at different temperatures essay

The effect of molecular weight on the rate of diffusion of potassium permanganate (kmno4), potassium dichromate (k2cr2o7) and methylene blue ¹ abstract the effect of molecular weight on the rate of diffusion was determined using the agar-water gel test. Study 113 lab exam 3 flashcards from lacy w on studyblue study 113 lab exam 3 flashcards from lacy w on studyblue eosin methylene blue agar (levine) - quadrant streak - complex, selective, differential a 21 plaques are recorded on an agar plate, you should use that number to calculate the overall phage count. Bio lab chp 3 study play in lab we measured diffusion rates of dye through an agar gel what two solutions were tested using the agar gel potassium permanganate is half the weight of methylene blue, what does this mean the lighter and smaller the molecule, the faster the diffusion rate.

  • Dipped a different wooden stick in the methylene blue crystals, held it so the end of the stick with the dye on it was up, held the petri dish upside down, and touched the dye covered stick to one side of each of the three agar petri dishes.
  • The effect of different molar mass on the diffusion on substances the effect of different molar mass on the diffusion on substances lunar-maius a gaerlan group 2 sec x – 9l august 15, 2012 abstract the effect of molecular weight on the rate of diffusion was assessed using agar-water gel test.
  • Experiments: osmosis and diffusion diffusion experiment – 1 aim: to demonstrate the process of diffusion requirements: water, beaker, potassium permanganate crystals procedure: 1 take a beaker 2 fill half of it with water 3 add a few crystals of kmno 4 (potassium permanganate) to it 4 observe what happens to the crystals.

Potassium permanganate, potassium dichromate, and methylene blue, agar – water gel was used three bottles with dropper containing the substances and a petri dish with agar. Calculation of diffusion distances and rates molecular weights methylene blue: 320 grams/mole yellow food coloring: 22 grams/mole lab report a single representative from each lab group will turn in a report for that group. Best answer: diffusion goes faster at higher temperatures because diffusion relies on the kinetic energy of molecules higher temp = higher kinetic energy = faster rate at which the molecules mix together your experiment should have had the hot solution turn purple coloured and the cold solution turn pink. Aspirate about 2-3 drops of water from the beaker every 30 seconds and place a drop of it in 2 different test tubes combine 3ml of calcium chloride (cacl2) solution and 2ml of methylene blue then seal with dried longganisa skin with rubber band cloudy) is evident in both test tubes.

diffusion rate of methylene blue on agar plates at different temperatures essay At the end of the 5-h incubation period, the viable count had declined from a starting value of 16×10 6 cfu ml −1 to undetectable on the selective agar plates mlsa and to a value of 63 cfu ml −1 on the non-selective agar plates na.
Diffusion rate of methylene blue on agar plates at different temperatures essay
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